temp and humidity for clones

When I talked to Jonah Hill a few weeks before SuperBad was released last year, I asked about a possible sequel. No one could go see it, and the talk would instantly go away of there being a sequel.” And of course, SuperBad was a big hit, grossing over $169 million worldwide and who knows how much on DVD. Seth Rogen told moviehole that the studio has been after them to do a sequel ever since the film was released nearly a year ago, but they’ve ultimately decided not to make a sequel. “They’re been wanting us to do a sequel to Superbad for so long – but we’ve held off,” Rogen said. “We eventually said No.” But Rogen isn’t against the idea of sequels all together, adding that he would love to make another Pineapple Express if all goes well.

“If it did well, and they gave us like $50 million dollars, as opposed to the $26M we got for this one, we could benefit from that.” And from what I’ve heard, Pineapple Express is already tracking pretty high. Everyone I know who has seen it has told me that people are going to love it. And I’m sure Sony will be asking for a sequel after opening weekend. Sequel Talk: Pineapple Express, Hulk 3, Sex and the City 2, Bad Boys 3. Posted on Wednesday, June 4th, 2008 by Peter Sciretta. Hollywood loves sequels, and the readers of movie blogs love to hear the early talk of them (even if it never develops into anything). So lets take a look at the recent sequel gossip going around the interwebs. Seth Rogen and James Franco are already talking about the possibility of the first comedy sequel produced by Judd Apatow – Pineapple Express 2 ?

“Yeah, I’m waiting for the sequel,” Franco revealed to MTV at Sunday’s Movie Awards. Rogen added, “Yep, we’ve talked about it.” When asked who will play the villain in the next Incredible Hulk movie (if the demand warrants a sequel), director Louis Leterrier says that Samuel Sterns (played by Tim Blake Nelson) is introduced in his film and of course, will eventually become The Leader. [comics2film] At the 2008 MTV Movie Awards, Will Smith told Hollyscoop that he has an idea for Bad Boys 3 . “We need Bad Boys 3, but Michael is too expensive now, he’s way too expensive.” Even series director Michael Bay admitted that it might never happen even though they’ve talked about doing another one. “We talk about it, but we all make too much money.” Meanwhile The Daily Mail claims that the producers of Sex and the City are “exercising the sequel option in all of the stars’ contracts. They want it to be a franchise and think they can stretch it over at least a trilogy.” But can you really believe the British tabloid paper’s sources? Выполните вход, чтобы сообщить о неприемлемом контенте. Release Date: 12 June 2013 (United States) THIS IS THE END follows six friends trapped in a house after a series of strange and catastrophic events devastate Los Angeles. As the world unravels outside, dwindling supplies and cabin fever threaten to tear apart the friendships inside. Eventually, they are forced to leave the house, facing their fate and the true meaning of friendship and redemption. Genre: Comedy Cast: James Franco, David Krumholtz, Danny McBride, Jay Baruchel, Directors: Seth Rogen, Evan Goldberg Writers: Seth Rogen, Evan Goldberg. The banana seed contains the embryonic plant that will develop into a seedling. The formation of the seed is part of the process of sexual reproduction, which involves the fertilization of an ovule (located inside the portion of the female flower called the gynoecium) by pollen produced by male flowers. In dry mature seeds, the hard, leaky outer layer of the coat (the outer integument) acts like a cage, while the thin inner layer of the coat (the inner integument), which is lined with a cuticle, is impermeable to water and provides an almost complete envelope surrounding the embryo and endosperm. Only wild species of bananas — and under certain circumstances cultivars that have residual fertility — produce seeds. The seed, like the one of the wild species, Musa balbisiana, has an extremely durable, wrinkled coat, the only opening of which is sealed by a shortened conical micropylar plug, also called an operculum (see figure). The seed coat consists of a thick hardened, but leaky, outer integument, and a thin inner integument, on the inner surface of which is a thick cuticle. Seeds of wild bananas are usually less than 1 cm in diameter [1] . The seed has two chambers: the larger one containing the endosperm and the embryo, and the smaller one the chalazal mass. Apart from a small discontinuity of parenchymatic tissue, the chambers are separated by the inner integument and cuticle. At the micropylar end, the inner integument projects into the micropylar canal [2] , forming a cap to the larger chamber. In Musa acuminata , the larger chamber occupies just over 50% of seed volume, while the smaller chamber, the micropylar plug and other outer integument material account for the remaining volume [3] [4] . While size, colour and shape varied, a study of 20 species of wild and edible bananas found this basic seed structure and anatomy in all of them [5] . The water content of fresh banana seeds ranges from 31 to 46%, falling to below 10% when seeds are dried at around 20 o C [5] .

The chalazal mass, which in fresh seeds was observed to be "full of water", shrivels upon drying [5] . The basic external requirements for germination are water, oxygen and an appropriate temperature. The germination of a seed begins with water uptake (imbibition), followed by embryo expansion along its axis through cell elongation, and ends with the breaking-through of the seed coat by the embryo root (radicle), then shoot (plumule). In the banana seed, the endosperm disappears within two weeks and within three weeks, the seedling has a lateral root system, and a prominent shoot [6] , in which the first leaves have elongated beyond the cotyledonary sheath [7] .

By the fourth week, the seedling is dependent on the root system for inorganic nutrition [6] . Water must enter the chamber containing the embryo and endosperm before germination can occur.

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