hydrogen peroxide seeds

Hydrogen peroxide soak for seeds?

I have read on some other threads that many gardeners are having great luck with soaking older seeds in good ol’ hydrogen peroxide solution, that soaking seeds in hydrogen peroxide can help to get through the hard shell and increase germination? Has anyone here had any luck with hydrogen peroxide and germination?

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I have used this method for many, many years. Works well.

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how long should brugmansia seed be soaked?

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How old seeds and what type would benefit from this? Does anyone know the science behind this? Does it need to be warm hydroperox? How long a soak?

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Well, i don’t know if it helps seedlings break through the seed coat, but I do know that soaking seeds in a 3% solution of hydrogen peroxide for 10-30 minutes, followed by at least 10 rinses in clean water will kill any pathogen growing on the outside of the seed. Germination appears increase after this sterilization because some seeds get attacked by pests as soon as germination begins and the embryo is destroyed before it even emerges from the soil. This is routinely done in plant research laboratories to prevent powdery mildew, mold etc. It also prevents damping off. You can also use a 3% solution of bleach, as long as the hypochlorite level is low: javex is an excellent brand. The cheaper bleaches are not as pure and won’t have the same effect. As for the hydrogen peroxide, I’d recommend using a food grade solution.

As for a ‘biological’ explaination, hydrogen peroxide is a plant signalling molecule (like ethylene) used by the plant to mediate pathogen and environmental stress responses. It also *MAY* help in root development. I’ve heard of some people actually watering or spraying plants with low doses of hydrogen peroxide to help plants overcome pathogen attack, or make them hardier if a pathogen make its way into the garden. Personally, I don’t like spraying my plants with too many chemicals, and if you do have a diseased plant, it is better to sacrifice it before it infects everything else. Plus, not much is known about hydrogen peroxide plant signalling yet, the hydrogen peroxide may stop pathogen attack, but what else is it telling your confused plant to do?

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albert_135 39.17°N 119.76°W 4695ft.

I took some honey locust Gleditsia (?) and scratched them with a file (My experience has been they may sometimes soak for a year without germination unless they go through some freeze/thaw cycles.) and I put some in a household peroxide soak and some in a bottled water soak. Both samples started to swell in about 12 hours. Those in the bottled water produced roots about 48-72 hours before those in the peroxide.

The unscrached controlls in water and in peroxide show no indication of doing anything as was expected.

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It could have been coincidence, but I spritzed the pot with Echinocactus polycephalus seeds in it (and everything else in the flat) with 0.3% hydrogen peroxide (the drugstore stuff diluted 1/10) and the next day the seeds started germinating. Okay, so nothing else in the flat has germinated yet, so it probably IS coincidence.

I have read on some other threads that many gardeners are having great luck with soaking older seeds in good ol' hydrogen peroxide solution, that soaking seeds in hydrogen peroxide can help to get through the hard shell and increase germination? Has anyone here had any luck with hydrogen peroxide an…

Role of H2O2 in pea seed germination


The imbibition of pea seeds with hydrogen peroxide (H2O2) increased the germination as well as the seedling growth, producing an invigoration of the seeds. We propose that H2O2 could acts as signaling molecule in the beginning of seed germination involving specific changes at proteomic, transcriptomic and hormonal levels. These findings have practical implication in the context of seed priming technologies to invigorate low vigour seeds.

Germination process is associated with many metabolic, cellular, and molecular events, coordinated by a complex regulatory network. The reactive oxygen species (ROS) production by germinating seeds has often been considered as a negative effect that might affect the germination process, but provided that their accumulation is tightly regulated by the balance between production and scavenging, these toxic molecules now appear as being beneficial for germination. 1

Strategies for improving the growth and development of crop species have been investigated for many years. Seed priming is a pre-sowing strategy to influence seed germination and seedling development by modulating pre-germination metabolic activity prior to emergence of the radicle and generally enhances germination rate and plant performance. 2 , 3 From a biochemical and molecular point of view, studying germination is difficult because a population of seeds does not complete the process synchronously. 4 Seed priming has been found as technology to enhance rapid and uniform emergence, and to achieve high vigour and better yields. This process generally causes faster germination and faster field emergence, which has practical agronomic implications, notably under adverse germination conditions. 5

Effect of ROS-Related Compound in Seed Germination and Seedling Growth

We have tested the priming effect of several compounds on pea seeds. The assayed compound can be divided in three major groups: directly involved in the antioxidative metabolism [hydrogen peroxide (H2O2), reduced and oxidized glutathione (GSH, GSSG) and ascorbic acid (ASC)]; compounds related with the amino acid cysteine [N-acetylcysteine (NAC), thioproline (TP) and L-2-oxo-4-thiazolidine-carboxylic acid (OTC)]; and compounds that trigger the systemic acquired resistance in plants [salicylic acid (SA) and its functional analog benzothiadiazole (BTH)].

The tripeptide thiol glutathione (GSH) is at the hub of the complex antioxidant networks of plant and animal cells, where it participates in the cellular redox signaling networks that influence growth, development and defense. 6 , 7 It has long been recognized that ASC also exert a strong influence on plant growth and development. 8 The GSH synthesis is regulated by cysteine availability, thus compounds increasing its contents could produce an increase of GSH levels. In this sense, OTC is an artificial cysteine precursor whereas TP is a proline analog that is converted by proline oxidase to N-formyl- l -cysteine which is presumably hydrolized to cysteine. 9

To carry out this work, pea seeds were imbibed for 24 h in dH2O or in the compounds described above at different concentrations. Seeds were then washed twice with dH2O and placed in Petri dishes with two layers of filter paper moistened with dH2O. Seeds were incubated at 25°C for 48 h in darkness, in a Cooled Incubator (MIR-153 Sanyo).

Figure 1 shows that, except H2O2, none of the assayed compounds had a positive effect on seed germination or seedling growth (measured as fresh weight and length). Exogenous H2O2 showed a priming effect in the germination of pea seeds in a concentration dependent-manner obtaining more vigorous seedlings, being 20 mM H2O2 the concentration that produced the best response in terms of growth ( Fig. 1 ). The increase in seedling growth by 20 mM H2O2 was also evident 24 h after imbibition ( Fig. 2 ). The priming effect of H2O2 was also noticeable at shorter times of imbibition ( Table 1 ). After 12 h of imbibition about 15% of seeds had germinated; however, no germination occurred at this time in seeds imbibed in water. At 24 h of imbibition, this percentage had reached nearly 75%, whereas control seed germination remained at low level ( Table 1 ). In preliminary experiments, we noticed that H2O2 concentrations higher than 20 mM (40 to 100 mM) also stimulated the germination rate after 24 h imbibition. However, at short-term of post-imbibition (6 h) we observed that these H2O2 levels induced a pronounced curvature as well as an abnormal growth of the radicle at 24 h and 48 h post-imbibition ( Fig. 2 ). In addition, H2O2 concentrations above 100 mM reduced the pea seeds germination rate (data not shown).

Interestingly, we observed a differential response depending on when H2O2 was supplied. When 5 mM H2O2 was added during the incubation in plates, after imbibition in dH2O, the percentage germination was similar to that of control seeds. In contrast, the presence of 10 or 20 mM H2O2 in Petri dishes produced a negative effect on germination ( Fig. 3 ).

Figure 3. Percentage of germination of pea seeds after imbibition in the presence of dH2O (0 h) and 48 h of incubation in plates in the presence of different H2O2 concentrations.

H2O2 Signaling during Seed Germination

Numerous recent works show now that ROS would play a key signaling role in the achievement of major events of seed life, such as germination or dormancy release. In fact, hydrogen peroxide, nitric oxide, hydroxyl radicals and superoxide radicals have been shown to accumulate during seed germination in various species. 1 Many works have reported that exogenous application of H2O2 can improve seed germination in many plant species. 10 – 12 The interplay between ROS and hormone signaling pathways lead to changes in gene expression or in cellular redox status that would play a role in the perception of environmental factors by seeds during their germination. 1 Recently we have shown that H2O2 coordinates the beginning of pea seed germination, acting as a priming factor that involves specific changes at proteome, transcriptome and hormonal levels, resulting in an acceleration of the germination process most probably due to invigoration of the seeds. 13 H2O2 would induce a MAPK-dependent decrease in abcisic acid (ABA) contents in the seed as well as the carbonylation of seed storage proteins, 13 favoring their mobilization, and some glycolytic enzymes that could stimulate the phosphate pentose pathway (oxPPP). 14 The oxPPP activation could provide NADPH for the thioredoxin system, involved in seed germination and seedling development. 15 Alternatively, H2O2 could act, directly or indirectly impairing the ABA transport from the cotyledon to the embryo inducing a decrease in ABA, stimulating the germination process. 13 Finally, the decrease in ABA could induce a MAPK-mediated decrease in the ethylene precursor 1-aminocyclopropane carboxylic acid, favoring epicotyl and radicle emergence by H2O2 treatment. 13

As mentioned above, the H2O2 treatment also stimulates the early growth of pea seedlings ( Table 1 ). Previously, we have described that the H2O2-induced increase in pea seedling growth was correlated with the induction of proteins related to plant growth, cellular signaling and cell cycle control (14–3-3 protein, profilin, proteasome, translationally-controlled tumor protein), as well as with a substantial decrease in the levels of the hormones ABA and zeatin-riboside (ZR). 16 Moreover, a decrease of a polypeptide with homology to an ABA-responsive protein was observed, suggesting an interplay between the redox state and plant hormones, orchestrated by H2O2, in the induction of proteins related to plant signaling and development during the early growth of pea seedlings. 16 The molecular mechanisms implicated in this differential response remain unclear. They are of considerable interest, notably in the context of improving crop yields by invigoration seed treatments both in commercial applications 2 and in developing countries. 17

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

Role of H 2 O 2 in pea seed germination Abstract The imbibition of pea seeds with hydrogen peroxide (H 2 O 2 ) increased the germination as well as the seedling growth, producing an ]]>